Phytochemical Screening
Phytochemical tests of the extract were performed as previously described [21, 22]. Mayer's reagent was used for alkaloids, ferric chloride reagent for phenolics, Molish test for glycosides, Biuret reagent for peptides, Mg-HCl reagent for flavonoids, Liebermann-Burchard reagent for steroids, and silver nitrate reagent for organic acids.