based on dual probe hybridization multifinder multiplex PCR using specific probes hybridize two adjacent improve the detection specificity affect the screening sensitivity, prone to false-negative results; The technology uses a generation sequencing platforms PCR products, such as ABI3130, ABI3500 so expensive reagents supplies; after detecting nucleic acid extraction process, double probe hybridization probe connection, PCR amplification, product analysis and other steps, the operation is more complicated, the operator of the technical requirements are very high need to master the use of generation sequencing platforms. [0008] Based on the principle of arm-PCR multiplex PCR technology, although operational automation and product solves the problem of pollution, but the technology into a larger, expensive consumables.