The ergothioneine content was analyzed following the method of Briefly, 0.5 g dried mushroom powder, 7 ml cold ethanolic extract [10 mM dithiotreitol (DTT) in ethanol,
100 lM betaine in ethanol, 100 lM 2-Mercapto-1-methyl-imidazole (MMI) in 70% ethanol]
3 ml deionized water (DI) in a 50 ml
sterile Falcon tube was blended for 10 s at medium speed in a Sorvall
Omni Mixer model 17,150 115 volt 5 amp (Newtown