Crystalline regions of cellulosic biomass are less susceptible to
enzymatic hydrolysis than amorphous regions (Zhu et al., 2008).
Therefore, a decrease in the amount of crystalline regions and an
increase in the amount of amorphous regions is helpful in order
to increase sugar yield of hydrolysis substrates. One way to reduce
crystallinity is via size reduction that provides mechanical impacts
on cellulosic biomass (Yoshida et al., 2008; Zhang et al., 2007; Vidal
et al., 2011).