A non-enzymatic approach towards cholesterol detection is presented here,exploiting the electro-
chemical non-enzymatic route of sensing which has a distinct advantage over other conventional
enzymatic processes.
Chemically converted Graphene modified with β-CD, being hydrophilic,electro-
active and highsurface are a material,provides a platform for the electrochemical detection of cholesterol
using Methylene Blue as redox indicator.Methylene Blue (MB) forms an inclusion complex withGrp-β-
CD and emerges as a cholesterol sensing matrix.MB molecule is replaced by cholesterol molecule and
moves out in the buffer solution,hence,detected electrochemically using Differential Pulse Voltammetric
(DPV) technique.
The sensing matrix is characterised using FT-IR and Ramanspectroscopy.Transmission
Electron Microscopy is carried out to study the morphology of functionalized graphenesheets.