2.4.1. Cellulase concentration
The samples were added to a set of aqueous solutions of various
cellulase concentrations, namely, 0, 5, 10, 15, and 20 g/L. The pH of
the treatment bath was regulated to 4.5 by using acetic acidesodium
acetate buffer solution. Each solution had a liquor ratio of
15:1. Heating was done at 45 C for 3 h.