A medium used for multiple shoot induction was Hyponex
medium [11] containing 3g/L (6.5N-6P-19K) Hyponex,
100g/L potato homogenate, 2g/L peptone, 2.5g/L sucrose,
1g/L activated charcoal and 2.5g/L gelrite. The medium was
supplemented with 0.45 or 2.25μM Thidiazuron (TDZ) or
4.44 or 8.88μM N6-benzyladenine (BA) or 2.32 or 4.65μM
kinetin (Kn) alone or in combinations with 4.52 or 13.56μM
2,4-dichlorophenoxyacetic acid (2,4-D). The pH of all media
was adjusted to 5.4 prior to adding gelrite and autoclaving.
Explants were cultured, in 240mL glass jars containing 50mL
of culture medium, for 15 weeks. All cultured were incubated
at 24±1°C and under a 16-h photoperiod at 35–40μmol⋅m-2⋅s-1
provided by cool-white fluorescent lamps.