Tissue culture of sprout of Kluai Hin is the method for propagation to get many
seedlings in the short period of time. The seedling can be promoted to farmers to grow as a
professional. Therefore, this experiment was studied of 6 medias for initial propagation of Kluai
Hin. The MS medium + 30 g/l table sugar was used as a basal medium that compared with
liquid and solid medium which added BA 5, 8 milligrams per liter or coconut water 450 ml per
liter. After culture for 35 days, it was found that a solid MS medium + 8 mg / l BA + 30 g/l table
sugar gave the maximum average number of shoots and the highest average length of shoots.