Khanom-jeen (fermented rice noodles) is a traditional fermented food of Thailand since ancient times. Generally, flavor and taste from khanom-jeen occur as by-products from bacterial natural fermentation. Lactic acid bacteria (LABs) are microorganisms that play important roles in khanom-jeen process. In order to maintain their consistency of product, therefore, homofermentative LABs were screened and selected for use as a starter culture. LABs from this study were isolated from broken rice, fermented rice flour and rice noodle. The 268 isolates from 50 samples were Gram-positive bacteria, could not produce catalase enzyme and gas. LABs isolates were tested for their properties basis on % lactic acid and exopolysaccharides production including their growth in litmus milk and their ability to hydrolyse protein and starch. Our LABs were identified after compiling the results from api 50 ®CHL test kit. The selected Lactobacillus crispatus FF206, a high lactic acid and amylase producing and Lactobacillus paracasei ssp. paracasei R190, a high protease but not bioamine producing LABs will be used as starter culture(s) for preparing khano