Production parameters were optimized as inoculum size 10 %
(volume per mass) and substrate:moisture ratio 1:1. Among different carbon sources supplemented,
glucose (0.04 g/g) showed enhanced enzyme production ((122±5) U/g). Supplementation
of different nitrogen sources (0.02 g/g) showed decline in enzyme production.
Optimum a-amylase enzyme activity was observed at 55 °C and pH=5. At 75 °C,
enzyme showed 90 % activity compared to 55 °C.