This paperpresentsquantificationoffiveanthraquinonederivatives(emodinglycoside,chrysophanol
glycoside,emodin,chrysophanolandphyscion)inrhizomesofhardenedmicro-propagated Rheum
emodi plantsusinghigh-performanceliquidchromatography(HPLC).Asepticshootcultureswereraised
using rhizomebuds.ShootmultiplicationoccurredinbothagargelledandliquidMurashigeandSkoog
(MS) mediumsupplementedwith10.0 mM 6-benzylaminopurine(BAP)and5.0 mM indole-3-butyric
acid (IBA).Rootedplantletsobtainedonplantgrowthregulator(PGR)-freemediumweretransferredto
soil with92%survival.HPLCanalysisrevealedthepresenceoffiveanthraquinonederivatives:emodin
glycoside,chrysophanolglycoside,emodin,chrysophanolandphyscioninrhizomesoftissueculture-
raised plants.Onlyemodinglycoside(1)andchrysophanolglycoside(2)werepresentin6-month-old
hardenedtissueculturedplants.Inaddition,theotherthreederivatives(emodin(3),chrysophanol(4)
and physcion(5))werealsodetectedafter9months.
Paperpresentsquantificationoffiveanthraquinonederivatives นี้ (emodinglycoside, chrysophanol
glycoside, emodin, chrysophanolandphyscion) เผยแพร่ inrhizomesofhardenedmicro Rheum
emodi plantsusinghigh-performanceliquidchromatography(HPLC)Asepticshootcultureswereraised
rhizomebuds ใช้ShootmultiplicationoccurredinbothagargelledandliquidMurashigeandSkoog
(MS) mediumsupplementedwith10มม. 0 6-benzylaminopurine (BAP) เวย์ and5.0 มม.อินโดล-3-butyric
กรด (อิบา)Rootedplantletsobtainedonplantgrowthregulator (PGR) -freemediumweretransferredto
ดิน with92% อยู่รอดHPLCanalysisrevealedthepresenceoffiveanthraquinonederivatives:emodin
glycoside, chrysophanolglycoside, emodin, chrysophanolandphyscioninrhizomesoftissueculture-
ยกพืชOnlyemodinglycoside (1) andchrysophanolglycoside (2) werepresentin6 เดือนอายุ
hardenedtissueculturedplantsInaddition, theotherthreederivatives (emodin (3) chrysophanol (4)
และ physcion(5)) werealsodetectedafter9months
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นี้ paperpresentsquantificationoffiveanthraquinonederivatives ( emodinglycoside chrysophanol Glycoside โมดิน ,
, ,
chrysophanolandphyscion ) inrhizomesofhardenedmicro ขยายพันธุ์ น้ำมูก emodi plantsusinghigh performanceliquidchromatography ( HPLC ) asepticshootcultureswereraised
ใช้ rhizomebuds . shootmultiplicationoccurredinbothagargelledandliquidmurashigeandskoog
( MS ) mediumsupplementedwith10 .0 มม. 6-benzylaminopurine ( BAP ) and5.0 มม. indole-3-butyric
acid ( IBA ) rootedplantletsobtainedonplantgrowthregulator ( pgr ) - freemediumweretransferredto
ดิน with92 % การอยู่รอด hplcanalysisrevealedthepresenceoffiveanthraquinonederivatives : โมดิน
ี้ chrysophanolglycoside โมดิน , , , chrysophanolandphyscioninrhizomesoftissueculture -
เลี้ยงพืชonlyemodinglycoside ( 1 ) andchrysophanolglycoside ( 2 ) werepresentin6 เดือน hardenedtissueculturedplants เก่า
และ theotherthreederivatives ( โมดิน chrysophanol ( 3 ) , ( 4 ) และ ( 5 )
physcion ) werealsodetectedafter9months .
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