Seeds and skin were removed using a
Langsenkamp type 161 Colossal Pulper and the resultant puree was stored at −20ºC. Ethanol
extracts were obtained from the puree.22 Briefly, blackberry puree (10 g) was treated under
sonication for 30 min with 25 mL of extraction solvent of ethanol containing 0.01% HCl (v/
v)..