2.5.1. Determination of total phenol content (TPC)
TPC in meat was determined using Folin–Ciocalteu reagent, following the modified method ofGutfinger (1981). To 0.1 mL supernatant was added 0.2 mL of the Folin–Ciocalteu, followed by the
addition of 3 mL sodium carbonate solution (5%). The reaction mixture was vortexed after incubation at 23 °C for 1 h. The absorbance
of phenolic content was measured spectrophotometrically (Hewlett
Packard, UV/visible light) at 765 nm. The quantification of phenolics
was based on the standard curve generated with the use of Gallic
acid and expressed as Gallic acid equivalent/g of meat