To the best of our knowledge, there is no report on plant regeneration from mature tissues of grove-grown citrus
trees. The objective of this study was to evaluate the possibil-
ity of in vitro plant regeneration from mature tissues of
grove-grown Thomson navel trees. Influence of different fac-
tors i.e. basal media, explant type and hormonal combina-
tions were comprehensively investigated and a reliable, re-
producible protocol for in vitro plant regeneration from
Thomson navel mature explants has been established. The
findings of this study would have an important impact on
producing shoots without a juvenile phase, accelerating the
subsequent evaluation of the regenerated plantlets.