To each tube, 0.5 ml of
the inoculum containing approximately 108
CFU/ml microorganisms was added. A control
test, containing inoculated broth supplemented
with only DMSO under identical
conditions with gentamicin, was also performed
as reference. All the tubes were
then incubated at 37 °C for 24 h and examined
for any evidence of growth.Statistical analysis
Tests were carried out in triplicate and
the results were calculated as mean ± SD.