Mavrik acaricide 20% (20 mL / Litter of water) was used in two forms, the first was applied as spraying directly on the honey bee combs including mature and immature stages of bees, while the second was used a stripe of cotton (2 x 20 cm) which impregnated for 24 hr. in Mavrik and held in one comb between the brood nest of each tested colony.