Strains FR95 and 99/R were grown in YPD broth (20.0 g/l
peptone, 10.0 g/l yeast extract, 20.0 g/l dextrose, Oxoid) for 72 h at
30 C. Cells were collected by centrifugation at 1100 g for 10 min at
4 C and then washed in distilled water. The washing procedure
was repeated 3 times. Preparation of spheroplasts and cell fractionations
were carried out as described by Riz