Samples of dahi, waste juices of apple, pineapple, mango, musambi, grape,
sugarcane, orange and jaggery were collected randomly from local markets of different areas
of Kolkata in sterile bottles and kept at 4°C. These samples were screened for isolation of
the yeast strains.
Isolation of yeast strains
The samples were serially diluted, plated on a selective medium, Martins Rose
Bengal agar Medium (33.6 gm /liter) and were incubated at 28°C for 48 hours. The colonies
appeared were further purified. Pure colonies were isolated and tested for further
characterization.
Morphological characterization
The strains were stained by lactophenol-cotton blue, carbol fuchsin and seen under
phase contrast microscope. The colonies were checked for their colour, outline and other