Nociceptive reaction towards thermal stimuli in hot plate test using mice is a well-validated model for the detection of opiate analgesic as well as several types of analgesic drugs from spinal origin (27, 28). Nociceptive pain inhibition was noticed highest at 120 minutes after administration of the extracts and the response time was increased from 6.1 seconds to 12.4 seconds that was comparable to the standard drug morphine where the response time was 16.1 seconds at 120 minutes of study. As the hot plate method is selective to screen centrally acting opiate analgesic drugs (29), the effect of the extract on this pain model indicates that it might be centrally acting.