Calibration curve was prepared using g-aminobutyric acid standard solution. Resistant starch was analyzed according to Goni, Garia-Alonso, and Saura-Calixto (1997). Around 50 mg of rice flour sample (dry weight) was added with 5 mL distilled water, adjusted pH to 2.0 and incubated with 0.2mLpepsin solution,whichwas prepared by dissolved pepsin enzyme with enzyme-to-protein ratio of 1:17 in 0.01 mol/L HCL, at 37 C for 1 h.