Four solvents such as petroleum ether, chloroform, ethanol and distilled water were used to prepare the extracts.
50gm of neem leaf powder was added in separated sterile conical flask with 50ml of each solvents, the content was
mixed well and kept in bioshaker for 24 hours. After 24 hours, the content in each flask was filtered using Whattman
no.1filter paper and then concentrated by evaporation.