The gamma irradiator used in this study is installed at the Gamma Irradiation Service and Nuclear Technology Research Center (GISC), Kasetsart University, Bangkok. It was manufactured by J.L.
Shepherd & Associates, under the Model MARK 1-30, Serial No. 1116, loaded with 4500 Curies of Cs-137 having a half-life of 30.12 years. The gamma irradiator was calibrated to irradiate 500 Gy of gamma rays to the seed lots for 82 minutes. The rate of 500 Gy was found to produce much variance while leaving over 60% of the surviving plants13. The M1 seeds were sown in the field surrounded by non-irradiated population as the control. The M2 seeds were bulk-harvested in each population. There were 7.76, 5.12, 11.02, and 8.72 kg from KPS 2, VC6468-11-1B, F1, and F2, respectively. The seeds were drilled in rows, after which the mutants were periodically observed right after germination. In each visit to the field, the mutant plants were marked with bamboo sticks for subsequent observations. Data were recorded on characters and number of the mutants. At maturity, each mutant plant was individually harvested. The remaining plants were bulk-harvested for M3 seeds and sown for further observation. Field cultural practices on this experiment were conducted based on standard management for mungbean grown in Thailand. Briefly, the seeds were drilled in rows of 50 cm apart at the rate of 20 seeds per a meter. Weeds were controlled by pre-emergence spraying of Imazathapyr at 250 g(ai)/ha. Late weeds were eradicated by hand weeding twice at 15 and 30 days after sowing. Insects were controlled by spraying with triazophose (Hostathion 40% EC) at the rate of 40 cc per 20 liters of water when the insect population was building up beyond the threshold level. Irrigation water was applied during the cropping season as needed
Irradiator แกมมาที่ใช้ในการศึกษานี้มีการติดตั้งที่ บริการฉายรังสีแกมมา และนิวเคลียร์เทคโนโลยีวิจัยศูนย์ (GISC), มหาวิทยาลัยเกษตรศาสตร์ กรุงเทพมหานคร มันถูกผลิต โดย J.L.Shepherd & Associates, under the Model MARK 1-30, Serial No. 1116, loaded with 4500 Curies of Cs-137 having a half-life of 30.12 years. The gamma irradiator was calibrated to irradiate 500 Gy of gamma rays to the seed lots for 82 minutes. The rate of 500 Gy was found to produce much variance while leaving over 60% of the surviving plants13. The M1 seeds were sown in the field surrounded by non-irradiated population as the control. The M2 seeds were bulk-harvested in each population. There were 7.76, 5.12, 11.02, and 8.72 kg from KPS 2, VC6468-11-1B, F1, and F2, respectively. The seeds were drilled in rows, after which the mutants were periodically observed right after germination. In each visit to the field, the mutant plants were marked with bamboo sticks for subsequent observations. Data were recorded on characters and number of the mutants. At maturity, each mutant plant was individually harvested. The remaining plants were bulk-harvested for M3 seeds and sown for further observation. Field cultural practices on this experiment were conducted based on standard management for mungbean grown in Thailand. Briefly, the seeds were drilled in rows of 50 cm apart at the rate of 20 seeds per a meter. Weeds were controlled by pre-emergence spraying of Imazathapyr at 250 g(ai)/ha. Late weeds were eradicated by hand weeding twice at 15 and 30 days after sowing. Insects were controlled by spraying with triazophose (Hostathion 40% EC) at the rate of 40 cc per 20 liters of water when the insect population was building up beyond the threshold level. Irrigation water was applied during the cropping season as needed
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