During small-scale fermentations, both L. rhamnosus IMC 501® and L.paracasei IMC 502® grew on buckwheat flour (BF) and oat bran (OB) and acidified them MRS medium, lowering the pH after 24 h of growth. The pH reduction was more pronounced for both strains with 6% of BF and OB respectively. SYNBIO® in mMRS with 6% BF gave the highest
pH reduction (data not shown). No pH drop was observed in mMRS medium without an energy source for all lactobacilli tested. As shown in Table 1 a significant growth of L. paracasei IMC 502® was detected on 6% of BF and 2% of OB with respect to the negative control. Interestingly, the probiotic growth in SYNBIO® samples was significantly higher on 4 and 6% of both BF and OB with respect to the control. Table 2 shows the time required to reach pH 4.6 ±0.03 during fermentation in whole milk.