MRS agar þ Sorbitol (MRS þ S)
MRS agar was prepared without glucose (10 g peptone; 8 g meat
extract; 4 g yeast extract; 2 g K2HPO4; 5 g sodium acetate; 2 g
ammonium citrate; 0.2 g magnesium sulfate; 0.05 g manganese
sulfate; 1 mL Tween 80; and 10 g agar dissolved in 1 L distilled
water) and autoclaved at 121 C for 15 min. For the sorbitol solution,
10 g D-sorbitol were dissolved in 100 mL distilled water, and
the solutionwas sterilized by filtration through 0.22 mmmembrane
filter (Millipore, S~ao Paulo, Brazil). Immediately before use, 10 mL
D-sorbitol solution was added for each 90 mL MRS agar (1% final
concentration) (Dave & Shah, 1996).
MRS agar þ Sorbitol (MRS þ S)MRS agar was prepared without glucose (10 g peptone; 8 g meatextract; 4 g yeast extract; 2 g K2HPO4; 5 g sodium acetate; 2 gammonium citrate; 0.2 g magnesium sulfate; 0.05 g manganesesulfate; 1 mL Tween 80; and 10 g agar dissolved in 1 L distilledwater) and autoclaved at 121 C for 15 min. For the sorbitol solution,10 g D-sorbitol were dissolved in 100 mL distilled water, andthe solutionwas sterilized by filtration through 0.22 mmmembranefilter (Millipore, S~ao Paulo, Brazil). Immediately before use, 10 mLD-sorbitol solution was added for each 90 mL MRS agar (1% finalconcentration) (Dave & Shah, 1996).
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