Microbiological analysis
Banana puree (10g) was serially diluted with sterilized peptone water (0.1%). For the standard plate counts, 1 mL of each dilution was pour plated in triplicate using plate count agar (Difco Laboratories, Detroit, MI) and incubated for 48h at 37°C. Yeast and mold counts were determined in triplicate on potato dextrose agar (Difco Laboratories, Detroit,MI), acidified with a 10% tartaric acid solution, and incubated for 5 days at 25°C.