• 10. The flocculating efficiency was calculated as follows Flocculating efficiency (%) as absorbance of blank control minus absorbance of supernatant divided by absorbance of blank control times 100.
• 11. Extraction Flocculant, measuring the growth of cells xn11and xn7 Centrifuged at 5000 Revolutions per minute substances for a period of 30 min It 2 parts supernatant to Measurement the absorbance at 660 nanometer sediment Soak them in cold ethanol at 4 degree celsius (to accelerate precipitation) After 12 hours the material centrifuged at 5000 Revolutions per minute /min for a period of 30 min. Dried at 40 degree celsius Will be Bioflocculant.
• 12. Effects of various salts, pH and thermal stability on flocculating efficiency
one. Study the effects of the infection xn11and xn7 included At different concentrations
two. Study the effects of positive ions included โพแทสเซียมไอออน โซเดียมไอออน แมกนีเซียมไอออน อะลูมิเนียมไอออน ไอร์ออนไอออน แคลเซียมไอออน
three. Study the effect of pH ranged 3 - 11
four. Study the effect of the temperature , at 20 – 40 degree celsius
• 13. The study of wastewater treatment using bioflocculant Dyes removal Mix the ingredients as follows (ชี้ๆ) Colour analysis with a colourimeter and Mix for 1 minute, then Set aside 10 minutes to precipitate 2 section Supernatant Colour analysis and Sediment Flocculating analysis
• 14. Removal of chromium in the wastewater in electroplating Mix the ingredients as follows Wastewater 50 ml Bioflocculant 1 ml Adjusted pH Chromium analysis with a AAS (Atomic Absorption spectroscopy) and Mix for 1 minute, then Set aside 10 minutes to precipitate 2 section Supernatant Chromium analysis and Sediment Flocculating analysis