Results: Seven different bacterial strains were isolated and screened for cellulase production
in submerged fermentation process. Among these seven tested bacterial strains; ASN2
showed maximum yield for cellulase production. This strain was further characterized
by biochemical and morphological tests and identified as Cellulomonas sp. ASN2.
Supplementation of glucose, peptone and cysteine to the fermentation medium are favored
enzyme secretion. The optimum pH and temperature for the activity of crude enzyme was
7.5 and 60oC, respectively. Metal profile of the enzyme indicated that Co2+ and Mn2+ are the
strong stimulators while Hg2+ and Fe2+ inhibited the activity of cellulase from cellulolytic
bacterial strain, Cellulomonas sp.ASN2.