The fractions
containing TherJR_0333 were concentrated and analyzed by UV–
visible spectroscopy and SDS–PAGE (12% gel) to check for the presence
of further contaminating proteins. Over time, the fraction
containing TherJR_0333 protein started to form a red precipitate.
Solubilization of this precipitate was achieved using 10 mM potassium
phosphate buffer (pH7.6) with 100 mM potassium chloride
and 0.05% of the non-ionic detergent n-Dodecyl-b-maltopyranoside
(DDM)