Gynura bicolor DC. which is widely used by the locals as natural remedies in folk medicine is consumed as a culinary cooked vegetable and believed to confer a wide range of benefits such as anticancer, anti-inflammatory, and possibly antihypertensive effects. This research has studied the total phenolic content and antioxidant capacity of crude extracts Gynura bicolor DC. The results demonstrated that ethyl acetate extract of Gynura bicolor DC. with the highest total phenolic content among the extracts generally showed the strong ability in scavenging DPPH radicals and metal chelating activity. Moreover, eight phenolic acids could be detected in both extracts in the range 20–174 mg per 100 g dry weight. Ferulic acid had higher phenolic acid content than any compound which was determined by the high-performance liquid chromatography (HPLC) method. These findings suggest that Gynura bicolor DC could be considered as a functional food and might provide antioxidative potential.