From this equation, the percent substitution yielding maximum final length (Sm) of larvae was
Sm=−0.01598(2(−0.000233))−1=34.2
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For DI values, no significant differences were found up to 50% substitution. A significant response to percent substitution was described by the equation (Fig. 1, P 0.05). The chemical score ( Table 4) showed that the first and second limiting amino acids were histidine and lysine for SPM and histidine and arginine for C. muelleri.