In the current study, we aimed to immobilize cholesterol oxidase (ChOx) on Layered Double Hydroxide
(LDH) for cholesterol sensing. Obtained results have shown that immobilized ChOx keeps its activity even
after several uses. However, some free radicals, such as hydrogen peroxide (H2O2), were generated
through the enzymatic activity. In this work, we investigated the effects of hexane extract from Acacia
salicina toward free radical scavenging activity. Hexane extract was an effective inhibitor of H2O2
generated by the enzymatic cholesterol/cholesterol oxidase catabolism (inhibition percentage 40%).
This activity could be attributed to the presence of phytosterols in this extract. The present study
demonstrates that extracts of A. salicina leaves are a potential source of antioxidant agents (most likely
sterols compounds), their antioxidant activity might be used in chemoprevention trials.