The aim of this study was to investigate the impact of Ag and Co
fed to earthworms (Lumbricus rubellus) both as nanoparticles and
ions through contaminated food on their health as well as on
microbial community of both soil and earthworm faeces. The
hypotheses to be tested were that the supply of Ag and Co can alter
the microbial community of earthworm faeces and that such
alteration in turn can induce changes in the soil microbial biomass,
activity and community structure. Furthermore, also earthworms
can be directly affected by contaminants so inducing a disorder in
their tissues. Finally, we hypothesised that Ag and Co could remain
within the body of earthworms also after a depuration period, thus
negatively affecting their health. In the
first part of the experiment,
biochemical soil properties, such as microbial biomass, its activity
and community structure, and parameters linked to earthworms
stress were investigated in order to contribute to the identification
and validation of biomarkers affected by contaminant exposure.
Phospholipid fatty acids (PLFAs) and total fatty acids (FAs) were
used as biomarkers of shifts in soil microbial community structure
and of earthworms stress, respectively. PLFAs were also deter-
mined on earthworm faeces to
find out possible relationships
between microbial communities within soil and earthworm
faeces.
The aim of this study was to investigate the impact of Ag and Cofed to earthworms (Lumbricus rubellus) both as nanoparticles andions through contaminated food on their health as well as onmicrobial community of both soil and earthworm faeces. Thehypotheses to be tested were that the supply of Ag and Co can alterthe microbial community of earthworm faeces and that suchalteration in turn can induce changes in the soil microbial biomass,activity and community structure. Furthermore, also earthwormscan be directly affected by contaminants so inducing a disorder intheir tissues. Finally, we hypothesised that Ag and Co could remainwithin the body of earthworms also after a depuration period, thusnegatively affecting their health. In thefirst part of the experiment,biochemical soil properties, such as microbial biomass, its activityand community structure, and parameters linked to earthwormsstress were investigated in order to contribute to the identificationand validation of biomarkers affected by contaminant exposure.Phospholipid fatty acids (PLFAs) and total fatty acids (FAs) wereused as biomarkers of shifts in soil microbial community structureand of earthworms stress, respectively. PLFAs were also deter-mined on earthworm faeces tofind out possible relationshipsbetween microbial communities within soil and earthwormfaeces.
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