Experimental design
Before the start of the study, a pilot experiment was conducted in order to determine the dosage of GT. Four female overweight sub- jects (age 37·5 (SD 13·1) years, BMI 25·8 (SD 1·6) kg/m2) partici- pated in this experiment, which consisted of three indirect calorimetry tests. The day before the test, the subjects’ food and caffeine intake were standardised by the investigators. On the day of the test, REE and substrate oxidation were measured by means of an open-circuit, ventilated-hood system with subjects, in the fasted state, lying supine for 30 min. The subjects then received a dose of one of three treatments: PLAC; low-dosage GT (50 mg caf- feine, 250mg catechins) or high-dosage GT (75mg caffeine, 375 mg catechins), together with a meal replacement drink (Slim·- Fastw ready-to-drink shake, French vanilla, 903 kJ; Unilever Best- foods Nederland BV, Rotterdam, The Netherlands). EE and substrate oxidation were measured for 180 min after ingestion of the treatment. We concluded that there was a significant increase in diet-induced thermogenesis with the combined intake of the meal replacement drink and high-dosage GT capsules, compared with low-dosage GT. It was therefore proposed that further long- term research on the thermogenic effects of GT should focus on the high dosage of tea catechins and caffeine.
 
Experimental designBefore the start of the study, a pilot experiment was conducted in order to determine the dosage of GT. Four female overweight sub- jects (age 37·5 (SD 13·1) years, BMI 25·8 (SD 1·6) kg/m2) partici- pated in this experiment, which consisted of three indirect calorimetry tests. The day before the test, the subjects’ food and caffeine intake were standardised by the investigators. On the day of the test, REE and substrate oxidation were measured by means of an open-circuit, ventilated-hood system with subjects, in the fasted state, lying supine for 30 min. The subjects then received a dose of one of three treatments: PLAC; low-dosage GT (50 mg caf- feine, 250mg catechins) or high-dosage GT (75mg caffeine, 375 mg catechins), together with a meal replacement drink (Slim·- Fastw ready-to-drink shake, French vanilla, 903 kJ; Unilever Best- foods Nederland BV, Rotterdam, The Netherlands). EE and substrate oxidation were measured for 180 min after ingestion of the treatment. We concluded that there was a significant increase in diet-induced thermogenesis with the combined intake of the meal replacement drink and high-dosage GT capsules, compared with low-dosage GT. It was therefore proposed that further long- term research on the thermogenic effects of GT should focus on the high dosage of tea catechins and caffeine.
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