hrcC deletion resulted in the loss of
T3S in AAC00-1, which was confirmed by lack of an HR when
AAC00-1ΔhrcC was infiltrated into tobacco leaves (Fig. 2A). In
contrast, AAC00-1 (wild-type) and AAC00-1ΔhrcCcomp induced a
typical HR on tobacco leaves (Fig. 2A). As expected, leaf infiltrations
with PBS did not yield an HR on tobacco. When infiltrated
into watermelon seedlings, AAC00-1 and AAC00-1ΔhrcCcomp
produced typical water-soaking and necrosis by 2 days post inoculation
(dpi) (Fig. 2B). In contrast, seedlings infiltrated with
AAC00-1ΔhrcC did not develop BFB symptoms and yielded results
that were comparable with seedlings inoculated with PBS
(Fig. 2B).