Oligofructose or ascorbic acid did not exert a protective effect on probiotic cultures during storage, but the juices showed viability of probiotic culture higher than 106 CFU/mL during 28 days of cold storage. The oligofructose content was maintained at appropriated concentrations (1.65e1.68 g/ 100 mL) during storage. It is possible to develop synbiotic orange juice added oligofructose (prebiotic) and probiotic cultures without changing the physicochemical characteristics and acceptance of pure juice and that may be cold stored for 28 days.