5HPLC/UV–vis chromatograms (450 nm) of collected fractions of the CCC sep-aration of the crude carrot extract with a) (III) -carotene b) (II) -carotene and c) (I)lutein. Conditions: polymeric C30column (250 × 4.6 mm i.d., 5 m particle size); lin-ear gradient from 100% solvent A: MeOH/MTBE/H2O (81:15:4, v/v/v) to 100% solventB: MeOH/MTBE/H2O (6:90:4, v/v/v) in 40 min; flow-rate: 1.0 mL/min.