In this context, the main aim of this work was to study the evolution of the hydrolysis of waxy maize starch catalyzed by -amylase from Bacillus licheniformis, and determine whether under the chosen conditions hydrolysis proceeds preferentially in the amorphous regions of starch. Moreover, in a case of a preferential action towards the less ordered regions of starch, another aimof this contribution was to determine the conditions required toisolate partially hydrolyzed starch granules with increased crystallinity.