Then, the cultures were grown for 72 h
in a static incubator at 30°C for yeast and 37°C for bacteria. At the
end of the fermentation period, 45ml of methanol was added to the
fermented product of 15 g of intact okara and the mixture was
shaken at 92 strokes/min for 60 min with reciprocal shaker, and
then filtered to obtain the culture filtrate (Ohno et al., 1995).