The food waste hydrolysate was then used as the nutrient source for dark fermentative hydrogen production by Biohydrogenbacterium R3. The best hydrogen yield of 52.4 mL H2/g food waste was achieved at food waste mass ratio of 5% (w/v). The modified Gompertz model could be used to describe the cumulative hydrogen production for the food waste hydrolysate. The results demonstrated a promising combined bioprocess for hydrogen production from food waste.