Determination ofFatty acid
Fatty acids were separated from the silkworm pupae oil by soxhlet extraction and maceration
extraction. The fatty acid composition of the oils given in Table 2 was relatively determined from GC
chromatograms. In silkworm oil comprised of 2 type fatty acid, saturated fatty acid and unsaturated fatty
acid. Unsaturated fatty acid; Alpha linolenic acid and linoleic acid are essential fatty acid was found that
in maceration extraction more than soxhlet extraction when compared with other varieties. Because of
maceration extraction was not heating the chemical compound stable more than soxhlet extraction. The
silk worm pupae oils obtained from maceration extraction of None-Ruesee varieties are the highest oleic
acid as a component of 30.17 % but less than avocado oil and 42.31 % alpha linolenic acid. And the oil
from soxhlet extraction of Khewsakon varieties are the highest oleic acid as a component of 38.82 % and
32.06 % alpha linolenic acid but more than avocado oil. These 2 types of fatty acid essential for human
body. It was comprise of phospholipids which are cell structure and reduce cholesterol in blood. The lack
of fatty acid effect on growth rate and skin ulcer. The human body can not produce this linoleic acid, so
human bodies get it from food. (Sappayatosok, 1988) Silkworm pupae have been oil more than soybean.
Soybean was comprised oil 18.7%. (Manit et al., 2000) However soybean has linoleic acid 51 % which
more than silkworm pupae but soybean has linolenic acid 7% which less than silkworm pupae (LG Wade,
2000). The silkworm pupae would be a good source of the functional fatty acid,α-linolenic acid. Thus,
making it potentially useful for food and cosmetic industry.