OsCyp2-P is localized both in cytosol and nucleus in rice
OsCyp2-P protein lacks any nuclear signal (NLS) and is predicted to be cytosolic. To confirm it, we carried out Western analysis of the subcellular protein fractions in rice (Fig. 2). The cytosolic marker enzyme aldolase and nuclear marker protein histone H3 showed predominance in cytosolic and nuclear fractions, respectively, establishing the purity of these fractions. However, both the cytosolic and nuclear fractions showed the presence of OsCYP2-P protein suggesting the predominance of this protein in both cytosol and nucleus. The presence of this protein in the cytosolic fraction is much higher as compared to the nuclear, suggesting its major involvement in the cytosolic events.