The trial was carried out in a Buffalo Farm in Amaseno
town (Frosinone, Italy) on 35 buffalo cows subdivided in 3 groups, homogeneous for parity and
time of calving: Group A, 11 animals, Control with farm standard diet; Group B, 12 animals,
with the same diet, added with 6g Biosaf/day to verify the Biosaf efficiency; Group C, 12 animals,
with the same diet, added with 60g Biosaf/day, to verify the Biosaf innocuousness.