2.5.2. Vapour diffusion method
Antibacterial activity of EO in vapour phase was evaluated by
disc volatilization method at three different volumes (10, 20 and
30 ml per disc). In brief, solidified medium was inoculated over the
surface of MHA/SDA with 0.1 ml of microorganism suspension. A
paper disc (diameter 9 mm, Sigma, Steinheim, Germany) was laid
on the inside surface of the upper lid and 10 ml of EO was placed on
each disc.