With excitation at 488 nm using an argon laser, the
CAF of microalgae was measured through a 635 20 nm long pass
filter and GAF via a 530 30 nm band-pass filter. Two dimensional
profiles of FSC against red fluorescence and green
fluorescence vs. red fluorescence were electronically plotted,
where the sorting windows were positioned at optimal areas
within the distribution of an algal cell cluster.