With the rapid increase of lubricant consumption, oil contamination becomes more serious. Biotreatment is an
important method to remove oil contamination with some advantages. In this study, acclimatized oilcontaminated soil and used lubricating oil were sampled to isolate lubricant-degrading strains by several
methods. 51 isolates were obtained and 24-well plates were employed to assess bacterial potential in highthroughput screening. The method was noted for the prominence of oil–water two-phase system with saving
chemicals, shortening cycles and lessening workloads. In order to decrease inaccuracy