Gram-positive bacteria Bacillus subtilis B068150 has been used as a biocontrol agent against
the pathogen Fusarium oxysporum cucumerinum. Cucumber was grown in three soils with strain
B068150 inoculated in a greenhouse for 90 days, and the colonization ability of strain B068150 in
cucumber rhizosphere and non-rhizosphere soils was determined. Changes in total bacteria and fungi
community composition and structures using denaturing gradient gel electrophoresis (DGGE) and
sequencing were determined. Colony counts showed that B068150 colonization in the rhizosphere
was significantly higher (p < 0.001) than in non-rhizosphere soils. Based on our data, the introduction
of B. bacillus B068150 did not change the diversity of microbial communities significantly in the
rhizosphere of three soils. Our data showed that population density of B068150 in clay soil had a
significant negative correlation on bacterial diversity in cucumber rhizosphere in comparison to loam
and sandy soils, suggesting that the impact of B068150 might be soil specific