Enzyme activity is expressed in nanokatals; 1 katal represents the conversion of 1 mol of substrate to product per second. Protein concentration was determined with the Bradford dye-binding assay (Bio-Rad), using BSA as a standard.
Enzyme activity is expressed in nanokatals; 1katal represents the conversion of 1 mol of substrate toproduct per second.Protein concentration was determined with the Bradforddye-binding assay (Bio-Rad), using BSA as a standard.