According to the results, the rubbing generated during the shoe wearing could produce the mechanical rupture and the biocide release from microcapsule. Futhermore, the rubbing and ironing tests carried out in the laboratory indicates that the durability of the gelatine-based microcapsules applied will depend on the part of the shoe (toe-cap, midfoot and heel) in which they are incorporated. The destruction of the gelatine-based microcapsules in toe and heel areas will be maximum meanwhile its durability will increase in midfoot area. These results were corroborated via shoe manufacturing trials. This kind of biodegradable microcapsule must be incorporated in the less-stressed shoe subjected areas, so that their most successful use would be to prepare antimicrobial-finished insoles which are included inside the shoe in the final step of shoemaking process.