The nutritive values of tropical fruit like coconut become interesting for consumers and useful for breeding program in Thailand. The two experiments were carried out in Completely Randomized Design with 3 replications. In the first experiment, the essential and non-essential amino acid content of liquid and solid endosperm of potential market varieties of coconut such young tender nut of Aromatic Green Dwarf (Nam Hom) and mature nut of Hybrid Nam Hom X Kathi (NHK) were studied. It was found that among 18 kinds of amino acids, the significantly differences were found on Aspatic acid, Cystine, Serine, Isoleucine, and Phenylalanine content while highly significant differences were found on Histidine and Methionine contents. The second experiment aims to determine the fatty acid composition of Virgin coconut oil of 20 coconut accession from coconut field gene bank of Thailand at Chumphon Horticultural Research Centre. The result showed that there were significantly differences on every single of eight fatty acid composition. Among eight fatty acid, the lauric acid is the most consumers concern and it was found that lauric acid content in VCO showed excellent (>49%) in the varieties of Chumphon Hybrid no.2, Sawi Hybrid no.1(MAWA), Hybid Chumphon 60, Renell Tall, Malayan Yellow Dwarf, and Thai Red Dwarf. These varieties were recommended for VCO processing