The raw pork LFD was then tested for its ability to detect pork gelatin.
Gelatin frompork is a heterogeneousmixture of water-soluble proteins
consisting largely of collagen extracted from acid-cured skin. A
modified approach was used (see Section 2), to ensure that the gelatin
was not in a gelled state. Pork gelatin-free gummy candy was spiked
with increasing amounts of pork gelatin, extracted, diluted 1:1 in LFD
running buffer, and then applied to the housed LFD (Method C). Using
this approach, an LOD of 2.5% contamination in candy was observed
(see Table 3). Using pork gelatin dissolved in phosphate buffered saline
(PBS) solution, we obtained an LOD of 0.1% pork gelatin, indicating that
extraction of pork gelatin from high gelatin-content foods such as
gummy candy was a limiting factor in the detectability of the target analyte
(matrix effect). Although 2.5% contamination is relatively high, the
current method for speciation of collagen relies on LC/MS technology